spoVE [2019-07-01 09:58:10]

SEDS family peptidoglycan glycosyltransferase, required for spore cortex peptidoglycan synthesis

locus: BSU15210

pI: 9.85

mw: 39.97 kDa

protein length: 366 aa

gene length: 1101 bp

function: spore cortex peptidoglycan synthesis

product: SEDS family peptidoglycan glycosyltransferase

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,590,317 1,591,417

The protein

Protein family

• [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|FtsW]/[protein|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|RodA]/[protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|SpoVE] family (according to Swiss-Prot)
• [SW|SEDS proteins] (shape, elongation, division, and sporulation) [Pubmed|27525505]

Paralogous protein(s)

• [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|FtsW], [protein|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|RodA]

Structure

• [PDB|6BAR] (from Thermus thermophilus,corresponds to aa 99... 357, 38% identity) [pubmed|29590088]

[SW|Localization]

• integral membrane protein, forespore [Pubmed|17981970]

Expression and Regulation

Operons

• genes: [gene|D48B7591467C493D2214D37AEA83643FE8C27BC6|murE]-[gene|4D3D4429E1F387BACB0D5A1084AA8DF179A16685|mraY]-[gene|0A9FF5A66F73464A780914A1424979CE753B3D9C|murD]-[gene|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE]-[gene|D52FDB5015B10E9B26D7FED5708457890C6EA43D|murG]-[gene|5E11616A51EE2CD1B8D3E33B18483521C504AD1F|murB]-[gene|B66FC02948DABBCE64DE2629D6E33E05647B4EEF|divIB]-[gene|5CDB49209BDCA86E09D6287DFC16B278D9A71379|ylxW]-[gene|081EA0CBCDA7ECDE8D128DD091C132BB194D19EB|ylxX]-[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]
  description: [Pubmed|1391053]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|8320223], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]
  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: repression, [Pubmed|15383836], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]

  regulation

  • expressed during vegatative growth [Pubmed|1391053]

• genes: [gene|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE]
  description: [Pubmed|1391053]

  regulation

  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]) [Pubmed|1391053]

• genes: [gene|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE]-[gene|D52FDB5015B10E9B26D7FED5708457890C6EA43D|murG]-[gene|5E11616A51EE2CD1B8D3E33B18483521C504AD1F|murB]-[gene|B66FC02948DABBCE64DE2629D6E33E05647B4EEF|divIB]-[gene|5CDB49209BDCA86E09D6287DFC16B278D9A71379|ylxW]-[gene|081EA0CBCDA7ECDE8D128DD091C132BB194D19EB|ylxX]-[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]
  description: [Pubmed|1391053]

  regulation

  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]) [Pubmed|1391053]

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

Biological materials

Mutant

• BKE15210 ([gene|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAGCAATCGACACCCCAA, downstream forward: _UP4_TAACGAATGTATTTCCAAGC
• BKK15210 ([gene|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAGCAATCGACACCCCAA, downstream forward: _UP4_TAACGAATGTATTTCCAAGC

References

• 20417640,17981970,8320223,1391053,15383836,26883633,27525505,29590088